2.3. Spatial structure of the triplet genetic code

In the first version of our page, and also in publications [4] the term «topological structure» of the genetic code has been used. This term reflects the relationship of the genetic code with the topology of the encoded protein structures. Not refusing from this term, in the sequel we will often use the term “spatial structure” of the genetic code, as more common. We already mentioned on the first page that some variants of spatial structure of the triplet code were offered. Detailed analysis of these variants is made in [6-8].  Let's continue the story about of the genetic code, having passed to detailed consideration of procedure of construction of spatial structure of the triplet genetic code and features of its structural organization.

2.3.1. Stages of construction of the spatial structure of the triplet genetic code

The spatial structure of the triplet genetic code has been constructed just as well as the doublet code, on the basis of the principle of single substitutions of bases in the triplets (the principle of single transitions).

 

Each base of the triplet can be connected by single transitions with the three other bases.

The result of this work was the structure that is isomorphic to the Boolean hypercube B6.

 

 

 

 

 

 

 

 

 

 

 

 

 

On this structure nine triplets originating from the ССС (3 C base substitution at each position of the triplet), and their relationships are shown in red.

 

It is L-structure of the Boolean hypercube. Triplets with substitutions in the first position are located at the left.

 

There is also a D-structure symmetric to it in which triplets with replacements in the first position are located on the right side of a hypercube.

 

The spatial structure of the genetic code containing transitions

C<-->G, U<-->A, C<-->U, G<-->A  and superimposed on it transitions C<-->A, U<-->G is a nine-dimensional simplex.

 

 

Fig. 9. The spatial structure of the triplet genetic code, which is isomorphic to the Boolean hypercube B6.

 

Let's try to understand this "abracadabra".

 

2.3.2. Features of the structural organization of the triplet genetic code

The structure of the triplet genetic code inherited several properties from the hypercube B6. In addition, it has its own, characteristic for a triplet code, properties, namely: single transitions, an arrangement of the triplets encoding the similar amino acids, Rumer’s transformation. We will consider them in more detail.

 

2.3.2.1. Properties of the structure of the triplet genetic code, inherited from the B6 hypercube

A. Longlines

Longlines

Triplets

 

 

 

 

 

 

 

The spatial structure of the triplet genetic code contains
7
longlines with different numbers of triplets:
1 triplet - in the I and VII
longlines
6 triplets - in II and VI
longlines
15 - in III-rd and V
longlines and
20 triplets - in the IV
longline

 

 

 

 

I

II

III

IV

V

VI

VII

 

1

6

15

20

15

6

1

 

Fig. 10. Longlined structure of the triplet genetic code.

B. Hierarchical Organization

 

 

а

 

 

 б

 

 в

Fig. 11. The hierarchical organization of the spatial structure of the genetic code.
a - two sets of 32 triplets:
М1 and М2; b - four subsets of 16 triplets: SM1, SM2, SM3, SM4; c - eight octets: : О1 - О8.

 

 

2.3.2.2. The specific properties of the spatial structure of the genetic code

A. The single transitions

 

In Figure 9 possibility of formation of three types of transitions for each of three bases of triplets is shown:

C<-->G, U<-->A between complementary bases (solid lines);

C<-->A, G<-->U between non-complementary bases (rare dotted line);

C<-->U, G<-->A transitions pyrimidine - pyrimidine and purine - purine (frequent dotted lline).

In practice, in a general form on the presented model it is possible to consider only two types of transitions: C<-->G, U<-->A и C<-->U, G<-->A. The third type of transitions C<-->A, G<-->U which is an imposition on the initial structure, for technical reasons in a general view is difficult to portray, therefore they are presented in an enlarged view in separate drawing (see transitions C<-->A, G<-->U).

 

Single transitions C<-->G, U<-->A

In the first position of the triplet these transitions occur between two sets (M1 и M2) and are shown in Figure 12 a. In the second position of the triplet they are formed in octets (Fig. 12 b). In the third position of the triplet, these transitions connect the adjacent octets within subsets (Fig. 12 c). If necessary, they can be seen in more detail, having clicked the underlined words in each line in the caption under the picture 12 or pictures themselves.

 

 

 a

 

 b

 

 

c

Fig. 12. Single transitions of C<-->G, U<-->A (solid lines).
a -
between the first triples bases (connect sets M1 and and M2);
b -
between the second bases (bind triplets within octets from top to bottom);
c -
between third bases (connect  triples within subsets).

Single transitions C<-->U, G<-->A

In the first position transitions of this type are observed within the octet of triplets down from right to left. They are shown in Figure 13 a. In the second position, on the contrary, they connect triplets in octets from top to bottom from left to right (Fig. 13 b). Finally, in the third position of triplet, these transitions connect subsets of triplets SM1 - SM2 and SM4 - SM3 (Fig. 13 c). If necessary, as for Figure 12, you can view them in more detail, having clicked the underlined words in each line in the caption under the picture 13 or pictures themselves.

 

 a

 

 b

 

 

 

c

Fig. 13. Single transitions of  C<-->U,т G<-->A  (frequent dashed line).
a -
between the first bases (connect triplets within octets from top to bottom from right to left);
b -
between the second bases (connect triplets within octets from top to bottom from left to right);
c -
between third bases (connect triplets of SM1 - SM2 and SM4 - SM3).

It is known that in the Boolean hypercube В6 each vertex is connected to six neighbors. Shown above, six different single transition, actually, make up the structure of the triplet genetic code, which is isomorphic to the Boolean hypercube В6.

 

Single transitions C<-->A, G<-->U (see separate Figure 14).

 

B. An arrangement of the triplets coding for similar amino acids

 

 

 

 

On the spatial structure of the genetic code triplets coding for similar amino acids form either a quartets, or a pair of triplets occurring in quartets.

The quartets of triplets which encode the same amino acid, derived from «red doublets», are marked in red (Fig. 15) and are located in the top part of each set:  M11 and M21.

 

Quartet of triplets, which are usually encoded by only two pairs of amino acids derived from the "dark blue doublets" are marked in blue (Fig. 15) and are located at the bottom of each of the sets of the hypercube: M12 and M22.

 

To see them in more detail, click the underlined capital letters of the text.

 

 

 

Fig. 15. An arrangement of quartets of triplets coding for similar amino acids on spatial structure of a genetic code.

C. Rumer’s transformation

 

 

 

 

 

 

 

 

 

 

 

 

 

As seen in Figure 16, the triplets of the groups
 
M11 and M21

are transformed in
triplets of groups
M12 and M22
by Rumer’s rule:

C <---> A, G <---> U,


occupying
in the hypercube symmetric position
  (Group of symmetry C2), for example:

ACC <---> CAA, GCC <---> UAA,

CCC <---> AAA, CCU <---> AAG etc.

 

 

Fig. 16. An arrangement of the triplets connected by Rumers’s transformation, on the spatial structure of the genetic code.

 

"Abracadabra" is over.

 

 

 

2.3.3. The spatial structure of the triplet genetic code: the results

 

It's time to admire the full spatial structure of the triplet genetic code, which is shown in Figure 17.

 

 

Fig. 17. The spatial structure of the triplet genetic code.

 

We summarize briefly all that we have considered in this section.

 

The spatial structure of the triplet genetic code can be constructed on the basis of single transitions of bases in triplets. This structure is isomorphic to the Boolean hypercube B6, which can be seen in Figure 17.

 

This structure, as seen in this figure, inherited from the B6 hypercube longlines structure (7 longlines) and a hierarchical organization (2 sets M1 and M2 of 32 triplets, 4 subsets SM1 – SM4 of 16 triples and eight octets O1 – O8).

 

The specific properties of this structure also observed in Figure 17 are:

- the presence of single transitions between the bases of triplets which are easy for tracking on this structure;

- the existence of two groups of triplets coding for similar amino acids, forming a quartet or a pair belonging to quartets (quartets, red and blue);

- transformation of two groups of triplets each other by rule C <--> A, G <--> U (Rumer’s transformation) and symmetry of these two groups in the structure.

 

 

For those who wish to think about the code the spatial structure of the triplet code on a white background is presented. It is conveniently to print on your printer.

 

 

The following questions arise:

 

- why the structure of the genetic code is isomorphic to the Boolean hypercube В6?

- why amino acids are assigned to the given triplets, instead of others?

- what explains the existence in the genetic code of two groups of amino acids, which are encoding by triplets related by Rumer’s transformation?

 

The theory of topological coding of proteins is trying to answer these and other questions (Section 3).

 

The story of how the spatial (topological) structure of a genetic code has been constructed,

read in V.A.Karasev's book "The Genetic code: new horizons"

 

 

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